Seems you have already done the basic troubleshooting tasks. Next step would be to tinker more with the NIC driver parameters, for example try manually setting speed and duplex (not auto).
Another thing you can try is resetting TCP/IP and Winsock, there are options to do that in the "File" menu in the TCP Optimizer. You can test using the Windows defaults settings, and after applying the TCP Optimizer "optimal" settings again, but resetting TCP/IP and Winsock ensures there were no other modifications from some other drivers/software.
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